荧光染料能够吸收特定波长区域的能量，然后在其他特定波长区域内重新发射该能量。一般来说，荧光染料会被高频率的照明（紫外UV、紫色或蓝色光谱区域内的波长）激发或吸收能量，然后会在频率较低的照明（绿色、红色或近红外光谱区域内的波长）中发射该能量。每种荧光染料都具备了能够高效吸收能量的波长，又称为峰值激发波长λ (nm)，以及能够将所吸收到的最多能量重新发射的对应波长，又称为峰值发射波长λ (nm)。
根据不同荧光染料的激发、发射波长，选择富爱其光电单独光学滤光片以及最大透射的波长将能够确保您获得清晰度最高的图像。下表为常见荧光染料、荧光团的吸收发射波长（实际峰值波长可能因不同应用而异，仅供参考。）
荧光团/荧光染料名称
吸收峰值
发射峰值
波长 (nm)
波长 (nm)
1,8-ANS
372
477
2-dodecylresorufin-lipid
582
595
4-methylumbelliferone
357
441
5-carboxy-2,7-dichlorofluorescein
500
525
5-carboxynapthofluorescein (pH 10)
555
615
5-FAM (5-carboxyfluorescein)
492
518
5-ROX (carboxy-X-rhodamine)
578
604
5-TAMRA (5-carboxytetramethylrhodamine, pH 7.0)
542
568
6-carboxyrhodamine 6G
525
555
6-HEX
533
559
6-JOE
520
548
6-TET
521
541
7-AAD (7-aminoactinomycin D)
548
648
7-amino-4-methylcoumarin (AMC)
346
442
7-hydroxy-4-methylcoumarin
360
442
ACMA (9-amino-6-chloro-2-methoxyacridine)
419
483
Acridine Orange (+DNA)
502
526
Acridine Orange (+RNA)
460
650
Acridine Yellow
470
550
Alexa Fluor® 350
343
441
Alexa Fluor® 405
401
422
Alexa Fluor® 430
431
541
Alexa Fluor® 488
499
520
Alexa Fluor® 500
503
525
Alexa Fluor® 514
518
543
Alexa Fluor® 532
534
553
Alexa Fluor® 546
556
572
Alexa Fluor® 555
553
565
Alexa Fluor® 568
579
603
Alexa Fluor® 594
590
618
Alexa Fluor® 633
631
647
Alexa Fluor® 635
633
648
Alexa Fluor® 647
653
668
Alexa Fluor® 660
663
691
Alexa Fluor® 680
679
702
Alexa Fluor® 700
696
719
Alexa Fluor® 750
751
776
Alexa Fluor® 790
782
805
AMCA (Aminomethylcoumarin)
350
448
AmCyan1
458
489
Amplex UltraRed peroxidation product (pH 7.5)
568
581
APC
651
660
APC (Allophycocyanin)
651
660
Aqua 431
430
478
AsRed 2
576
592
ATTO 390
392
477
ATTO 425
436
484
ATTO 465
453
507
ATTO 488
500
525
ATTO 495
495
527
ATTO 520
516
538
ATTO 532
501
545
ATTO 550
553
576
ATTO 565
563
592
ATTO 590
594
624
ATTO 594
601
624
ATTO 610
614
634
ATTO 620
618
642
ATTO 633
629
658
ATTO 635
635
658
ATTO 647
644
670
ATTO 647N
645
668
ATTO 655
663
683
ATTO 680
680
700
ATTO 700
699
719
ATTO 725
727
754
ATTO 740
740
764
AzamiGreen
494
505
BCECF (pH 5.5)
481
518
BCECF (pH 9.0)
502
528
BD Horizon V450
406
449
BD Horizon V500
410
500
BFP (EBFP)
380
440
BOBO™-1
461
484
BOBO™-3
570
605
BODIPY
505
513
BODIPY 581/591
588
597
BODIPY 630/650-X
625
641
BODIPY 650/665-X
646
664
BODIPY FL
505
512
BODIPY FL-MeOH
502
511
BODIPY R6G
528
547
BODIPY TMR-X
544
573
BODIPY TR-X (MeOH)
544
573
BODIPY TR-X phallacidin
590
621
BO-PRO-1
461
482
BO-PRO-3
574
604
BUV395 - BD Horizon Brilliant™ Ultraviolet 395
348
395
BV421 - BD Horizon Brilliant™ Violet 421
407
421
BV480 - BD Horizon Brilliant™ Violet 480
440
479
BV510 - BioLegend Brilliant™ Violet 510
405
510
CAL Fluor® Gold 540
522
544
CAL Fluor® Orange 560
537
558
CAL Fluor® Red 590
569
590
CAL Fluor® Red 610
590
610
Calcein
494
514
Calcein Blue
494
514
Calcium Crimson
589
609
Calcium Green-1
507
529
Calcium Orange
589
609
Calcofluor White
351
444
Carboxynaphthofluorescein
599
674
Cascade Blue™
401
419
Cascade Yellow™
399
549
CellTrace BODIPY TR methyl ester
598
625
CellTrace calcein violet
400
452
CellTracker Red CMTPX
586
614
CellTracker Violet BMQC+GSH
406
526
Cerulean
434
473
CFP (ECFP)
433
475
CFP2
490
510
ChlorophyllA
480
650
Cy2™
492
507
Cy3.5™
578
591
Cy3™
554
566
Cy5.5™
672
690
Cy5™
649
666
Cy7™
753
775
CyPet
435
477
CyQuant Cell Proliferation Assay
502
523
CyQUANT GR-DNA
502
523
DAF-FM-NO
495
519
Dansyl cadaverine
334
526
DAPI
359
461
Dapoxyl
373
579
DDAO
648
657
DEAC
432
472
Dendra2 (Green)
491
507
Dendra2 (Red)
554
569
DHR (Dihydrorhodamine 123)
507
529
Di-8-ANEPPS
467
631
DiA
457
586
DiD
647
669
DiD (DiIC18(5)) - Lipophilic Tracer
648
670
DiFMU-pH 3.0
319
450
DiFMU-pH 9.0
358
450
DiI
551
569
DiO
475
500
DiR
750
782
DM-NERF (pH 4.0)
494
530
Dronpa
502
516
DsRed
559
583
DsRed-Express
556
584
dTomato
556
582
DY-415
418
470
DY-505-Phalloidin
503
530
DY-530
537
560
DY-590
582
599
DY-630
634
656
DY-635
646
670
DY-675
675
699
DY-781
784
802
DyLight 350
351
434
DyLight 405
398
420
DyLight 488
493
518
DyLight 549
553
569
DyLight 594
593
618
DyLight 633
620
649
DyLight 649
651
673
DyLight 680
676
705
DyLight 750
751
778
DyLight 800
777
794
ECFP
435
475
ecliptic pHluorin pH5.5
473
507
ELF 97
343
536
Emerald
491
511
Eosin
525
546
ER-Tracker™ Blue-White DPX
372
557
Ethidium bromide
518
603
Ethidium homodimer
527
617
Ethyl-p-dimethylaminobenzoate
309
330
evoglow-Bs1
449
496
FITC (Fluorescein)
495
519
FlAsH-CCPFCC
511
530
Fluo-3
506
527
Fluo-4
494
516
Fluorescein dextran
501
524
Fluorescein-pH 8.0
489
517
Fluoro-Emerald
494
518
Fluoro-Gold™
365
418
FluoSpheres Blue fluorescent microspheres
356
414
FluoSpheres Crimson fluorescent microspheres
620
646
FluoSpheres Dark Red fluorescent microspheres
656
683
FluoSpheres Orange fluorescent microspheres
540
559
FluoSpheres Red fluorescent microspheres
577
607
FluoSpheres Yellow-Green fluorescent microspheres
503
514
FM 1-43
473
579
FM 4-64
505
725
Fura Red™ (high pH)
572
657
Fura-2 (calcium bound)
336
505
Fura-2 (calcium free)
366
515
GFP (EGFP)
489
511
GFPuv
395
509
Gold 525
521
551
Gold 550
551
564
Green 496
496
520
Green 500
501
524
HcRed1
588
618
HCS CellMask Red cytoplasmic
622
644
HCS LipidTOX Deep Red neutral lipid stain
635
652
HCS LipidTOX Green neutral lipid stain
498
507
HCS LipidTOX Green phospholipidosis
504
536
HCS LipidTOX Red neutral lipid stain
582
616
HCS LipidTOX Red phospholipidosis
584
608
HiLyte Fluor™ 405
403
428
HiLyte Fluor™ 488
497
526
HiLyte Fluor™ 555
550
566
HiLyte Fluor™ 594
592
616
HiLyte Fluor™ 647
649
673
HiLyte Fluor™ 680
688
702
HiLyte Fluor™ 750
750
778
Hoechst 33258
352
455
Hoechst 33342
352
455
Hoechst 34580
392
440
Hypericin
600
603
ICG
768
807
indo-1
346
479
IRDye 680 NHS Ester
683
710
IRDye 700DX
689
699
IRDye 700Phosphoramidite
691
699
IRDye 800 CW-MeOH
778
801
IRDye 800CW
775
792
IRDye 800CW-NHS Ester
775
792
IRDye 800Phosphoramidite
257
809
IRDye 800RS
787
812
JC-1
499
525
JO-JO-1, JO-PRO-1
530
544
Keima(pH)
445
625
KFP-Red
575
599
KusabiraOrange
549
564
LDS 751
560
711
LIVE-DEAD Fixable Aqua Dead Cell Stain
367
526
LIVE-DEAD Fixable Blue Dead Cell Stain
343
441
LIVE-DEAD Fixable Far Red Dead Cell Stain
653
668
LIVE-DEAD Fixable Green Dead Cell Stain
498
525
LIVE-DEAD Fixable Near-IR Dead Cell Stain
752
776
LIVE-DEAD Fixable Red Dead Cell Stain
595
613
LIVE-DEAD Fixable Violet Dead Cell Stain
403
455
LOLO-1
568
580
Lucifer yellow
428
544
LysoSensor Blue
374
424
Lysosensor Blue DND-192
249
424
LysoSensor Green
448
502
LysoTracker Blue
373
422
LysoTracker Green
501
509
LysoTracker Red
573
592
LysoTracker Red DND-99 (L-7528)
577
589
LysoTracker Yellow HCK-123
488
565
Magnesium Green
507
531
Magnesium Orange
550
574
mApple
566
594
Marina Blue
363
461
mBBr+GSH
394
490
mCherry
587
610
Merocyanine 540
559
579
mHoneyDew
478
562
MitoTracker™ Deep Red
641
662
MitoTracker™ Green
490
512
MitoTracker™ Orange
551
575
MitoTracker™ Red
578
598
mKate2
588
633
mKO
549
559
Monochlorobimane
388
482
mOrange
546
562
mOrange2
551
565
mPlum
589
649
mRFP
555
583
mRFP1
584
700
mRuby
558
605
mStrawberry
574
596
mTangerine
568
585
mTFP1
462
492
mWasabi
493
509
Naphthalene
275
322
NBD
460
528
NBD-X (MeOH)
467
538
NeuroTrace 500/525 Green Fluorescent Nissl Stain
495
524
Nile blue-EtOH
631
660
Nile Red
551
579
Nile red-phospolipid
553
637
Nile red-triglyceride
510
583
OFP
547
567
Orange 552
552
574
Oregon Green™ 488
498
526
Oregon Green™ 514
513
532
Pacific Blue™
404
455
Pacific Orange
400
551
PerCP
490
677
pHrodo™, succinimidyl ester
560
587
Phycoerythrin (PE)
565
575
Phycoerythrin (R-Phycoerythrin)
565
575
PicoGreen®
502
522
PKH67
488
500
POPO-1
433
457
POPO-3
533
574
PO-PRO-1
433
456
PO-PRO-3
539
570
Propidium Iodide (PI)
305
617
Pro-Q Diamond
556
583
Pro-Q Emerald
289
530
Purine
272
405
Qdot® 525 Nanocrystals
300
525
Qdot® 545 Nanocrystals
300
543
Qdot® 565 Nanocrystals
300
564
Qdot® 585 Nanocrystals
300
588
Qdot® 605 Nanocrystals
300
602
Qdot® 625 Nanocrystals
300
621
Qdot® 655 Nanocrystals
300
654
Qdot® 705 Nanocrystals
300
702
Qdot® 800 Nanocrystals
300
792
Quasar® 570
548
566
Quasar® 670
647
666
Quasar® 705
688
706
Quinine sulfate
349
461
ratiometric pHluorin pH5
500
509
ReAsH-CCPGCC
592
606
Red 580
580
603
Red 650
649
665
Resorufin
571
584
Rhod-2
553
577
Rhodamine 110
497
519
Rhodamine 123
507
529
Rhodamine 6G
529
551
Rhodamine B
542
564
Rhodamine Green
497
523
Rhodamine phalloidin
558
575
Rhodamine Red-X
573
591
Rhodol Green
497
524
Rose Bengal
559
570
R-phycoerythrin (PE)
565
576
rsGFP (red shifted GFP, S65T)
498
516
sgBFP™
387
451
sgGFP™ (super glow GFP)
472
506
Sirius
355
424
SNARF (carboxy) 488 Excitation pH6
548
586
SNARF (carboxy) 488 Excitation pH9
576
638
SNARF (carboxy) 514 Excitation pH6
549
587
SNARF (carboxy) 514 Excitation pH9
576
638
SNARF-1 488nm (ph 6.0)
548
586
SNARF-1 488nm (ph 9.0)
576
638
SNARF-1 514nm (ph 6.0)
549
587
SNARF-1 514nm (ph 9.0)
576
638
Sodium Green
507
532
SpectrumAqua
434
481
SpectrumBlue
405
449
SpectrumFRed (Far Red)
650
673
SpectrumGold
530
556
SpectrumGreen
497
538
SpectrumOrange
554
587
SpectrumRed
587
615
Sulforhodamine 101-EtOH
578
593
SYBR Gold nucleic acid gel stain-DNA
496
539
SYBR Green I nucleic acid gel stain-DNA
498
522
SYBR Safe DNA gel stain-DNA
509
526
SYPRO Ruby protein gel stain
469
631
SYTO 11
506
525
SYTO 13
448
506
SYTO 16
489
520
SYTO 17
619
638
SYTO 45
451
485
SYTO 59
622
644
SYTO 60
650
681
SYTO 61
618
650
SYTO 82
540
559
SYTO 9
483
500
SYTO Red Fluorescent Nucleic Acid Stains 60
655
675
SYTO Red Fluorescent Nucleic Acid Stains 62
655
675
SYTO Red Fluorescent Nucleic Acid Stains 63
655
675
SYTO RNASelect geen fluorescent cell stain
503
527
SYTOX Blue
444
470
SYTOX Green-DNA
504
524
SYTOX Orange
547
570
SYTOX Red-DNA
640
658
TagBFP
402
457
TagCFP
458
480
TagGFP2
482
505
TagRFP
556
583
TagYFP
508
524
Tetramethylrhodamine dextran
554
582
Texas Red dextran
592
613
Texas Red DHPE
584
608
Texas Red®
592
614
Texas Red-X®
595
613
ThiolTracker Violet+GSH
404
526
TO-PRO-1
515
531
TO-PRO-3
642
657
TOTO-1
514
531
TOTO-3
642
661
TRITC (Tetramethylrhodamine)
552
578
TRITC (Tetramethylrhodamine) - "reddish" appearance
545
620
Tryptophan
295
340
Tryrosine
253
394
TurboFP602
574
601
TurboFP635(Katushka)
591
638
TurboGFP
482
503
TurboRFP
553
573
TurboYFP
525
538
Venus
516
528
VIC
530
550
Vybrant DyeCycle Green
506
534
Vybrant DyeCycle Orange
519
563
Vybrant DyeCycle Violet
369
437
wtGFP (wild type GFP, non-UV excitation)
474
509
X-Rhod-1 Indicator
580
601
YFP (yellow GFP)
513
530
YO-PRO-1
491
506
YO-PRO-3
613
629
YOYO-1
491
508
YOYO-3
611
631
ZsYellow1
529
539
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